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6X His tag antibody [GT161]

Anti-6X His tag antibody [GT161] used in Western Blot (WB). GTX628921
Anti-6X His tag antibody [GT161] used in Western Blot (WB). GTX628921
Anti-6X His tag antibody [GT161] used in Western Blot (WB). GTX628921
Anti-6X His tag antibody [GT161] used in ELISA (ELISA). GTX628921
Anti-6X His tag antibody [GT161] used in Western Blot (WB). GTX628921

Cat. No. GTX628921

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT161

Isotype

 IgG2a

Application

 WB, ELISA

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all 6X His tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ELISA 1:1000-1:10000
Not tested in other applications.

Positive Control

His-tagged proteins

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to 6X His tag

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888083

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

6X His tag antibody , His antibody , 6x His antibody , 6x his tag antibody , His Tag antibody , 6xHis Tag antibody , 6 x His antibody

Research Area

DATA IMAGES

Anti-6X His tag antibody [GT161] used in Western Blot (WB). GTX628921

GTX628921 WB Image

6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis.
A. Non-induced cell lysate from E. coli expressing His-tagged proteins
B. IPTG-induced cell lysate from E. coli expressing His-tagged proteins
12% SDS-PAGE
6X His tag antibody [GT161] (GTX628921) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-6X His tag antibody [GT161] used in Western Blot (WB). GTX628921

GTX628921 WB Image

6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis.
Lane A and B were IPTG-induced cell lysate from E. coli expressing different GST-tagged proteins.
Lane C to H were IPTG induced cell lysate from E. coli expressing different His-tagged protein.
12% SDS-PAGE
6X His tag antibody [GT161] (GTX628921) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-6X His tag antibody [GT161] used in Western Blot (WB). GTX628921

GTX628921 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6X His tag antibody [GT161] (GTX628921) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-6X His tag antibody [GT161] used in ELISA (ELISA). GTX628921

GTX628921 ELISA Image

6X His tag antibody [GT161] detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 0.5 μg/ml 6X His tag antibody [GT161] (GTX628914). Bound Abs were further detected by using HRP-conjugated goat anti-mouse IgG (GTX213111-01) with HRP substrates.

Anti-6X His tag antibody [GT161] used in Western Blot (WB). GTX628921

GTX628921 WB Image

6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis.
A. 2 μg GST-recombinant protein
B. 2 μg His-recombinant protein
C. 1 μg His-recombinant protein
D. 0.5 μg His-recombinant protein
E. 0.1 μg His-recombinant protein
12% SDS-PAGE
6X His tag antibody [GT161] (GTX628921) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Lodato P.B. et al. FEMS Microbiol Lett 2017; (Epub) Effect of RNase E deficiency on translocon protein synthesis in an RNase E-inducible strain of enterohemorrhagic Escherichia coli O157:H7.
Application : WB
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations